Probing DNA sequences in solution with a monomer-excimer fluorescence color change.
The use of a simple fluorescent nucleoside analogue in detection of point mutations by hybridization in solution is described. Pyrene is placed at 3' and 5' ends of a pair of oligodeoxynucleotide probes via a phosphoramidite derivative of deoxyribose with this fluorophore attached at the 1' position...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147755/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9685497/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.16.3789 |
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