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Discovery of estrogen-responsive genes using an improved method which combines subtractive hybridization and PCR.
Here we describe a reliable method for isolating genes that are differentially expressed in two cell populations. The method is a combination of subtractive hybridization and PCR. Among many improvements to previously described methods is the incorporation of a new technology into the procedure whic...
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Autori principali: | , , |
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Natura: | Artigo |
Lingua: | Inglês |
Pubblicazione: |
1998
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Soggetti: | |
Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC147722/ https://ncbi.nlm.nih.gov/pubmed/9671829 |
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