Tailing cDNAs with terminal deoxynucleotidyl transferase in RT-PCR assays to identify ribozyme cleavage products.

Polytailing a cDNA with terminal deoxynucleotidyltransferase (TdT) results in the addition of a homopolymeric sequence at its 3'-end. Here we describe the use of tailing in competitive RT-PCR assays to evaluate cleavage efficiency of ribozymes. Using a system that perfectly mimics intracellular...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Albuquerque-Silva, J, Houard, S, Bollen, A
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1998
Gaiak:
Research Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC147666/
https://ncbi.nlm.nih.gov/pubmed/9628937
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