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An alternative PCR assay for quantifying mitochondrial DNA in crude preparations.

A method is described for the quantification of mitochondrial DNA present in crude biological preparations. A known copy number of a standard is amplified in the presence of inactivated target DNA so as to determine the overall efficiency of the PCR process in a particular sample. In this way any in...

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Detalhes bibliográficos
Main Authors: Koekemoer, T C, Downing, T G, Oelofsen, W
Formato: Artigo
Idioma:Inglês
Publicado em: 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC147619/
https://ncbi.nlm.nih.gov/pubmed/9592176
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