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Simple, efficient protocol for enzymatic synthesis of uniformly 13C, 15N-labeled DNA for heteronuclear NMR studies.

The use of uniformly 13C,15N-labeled RNA has greatly facilitated structural studies of RNA oligonucleotides by NMR. Application of similar methodologies for the study of DNA has been limited, primarily due to the lack of adequate methods for sample preparation. Methods for both chemical and enzymati...

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Udgivet i:Nucleic Acids Res
Principais autores: Masse, J E, Bortmann, P, Dieckmann, T, Feigon, J
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1998
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147604/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9592146/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.11.2618
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