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Confocal imaging of [Ca(2+)] in cellular organelles by SEER, shifted excitation and emission ratioing of fluorescence
Intracellular calcium signals regulate multiple cellular functions. They depend on release of Ca(2+) from cellular stores into the cytosol, a process that appears to be tightly controlled by changes in [Ca(2+)] within the store. A method to image free [Ca(2+)] within cellular organelles was devised,...
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Main Authors: | , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Blackwell Science Inc
2005
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1474212/ https://ncbi.nlm.nih.gov/pubmed/15946962 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1113/jphysiol.2005.087973 |
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