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Confocal imaging of [Ca(2+)] in cellular organelles by SEER, shifted excitation and emission ratioing of fluorescence

Intracellular calcium signals regulate multiple cellular functions. They depend on release of Ca(2+) from cellular stores into the cytosol, a process that appears to be tightly controlled by changes in [Ca(2+)] within the store. A method to image free [Ca(2+)] within cellular organelles was devised,...

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Detalhes bibliográficos
Main Authors: Launikonis, Bradley S, Zhou, Jingsong, Royer, Leandro, Shannon, Thomas R, Brum, Gustavo, Ríos, Eduardo
Formato: Artigo
Idioma:Inglês
Publicado em: Blackwell Science Inc 2005
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1474212/
https://ncbi.nlm.nih.gov/pubmed/15946962
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1113/jphysiol.2005.087973
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