A subtractive hybridisation method for the enrichment of moderately induced sequences.
Moderately induced genes often escape detection in conventional subtraction hybridisation cloning. Here a modification of a phagemid subtraction protocol is described that overcomes this problem. The protocol uses low ratio hybridisation of driver to target sequences to allow enrichment of the seque...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147408/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9469851/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.5.1359 |
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