Sensitive detection of p53 gene mutations by a 'mutant enriched' PCR-SSCP technique.
For the rapid and sensitive detection of p53 'hot spot' mutations, we combined polymerase chain reaction based single-strand conformational polymorphism (PCR-SSCP) analysis with sequence specific-clamping by peptide nucleic acids (PNAs) in a one-step reaction tube protocol. For this purpose, we desi...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147406/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9469850/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.5.1356 |
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