Codon-based mutagenesis using dimer-phosphoramidites.
A new approach for the synthesis of randomized DNA sequences containing the 20 codons corresponding to all natural amino acids is described. The strategy is based on the use of dinucleotide phosphoramidite building blocks within a resin-splitting procedure. Through this protocol, a minimal number of...
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| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1998
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147399/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9469829/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.5.1223 |
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