Cloning and analysis of the four genes coding for Bpu10I restriction-modification enzymes.
The Bpu 10I R-M system from Bacillus pumilus 10, which recognizes the asymmetric 5'-CCTNAGC sequence, has been cloned, sequenced and expressed in Escherichia coli . The system comprises four adjacent, similarly oriented genes encoding two m5C MTases and two subunits of Bpu 10I ENase (34.5 and 34 kDa...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1998
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147350/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9461472/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.4.1084 |
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