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Cloning and analysis of the four genes coding for Bpu10I restriction-modification enzymes.

The Bpu 10I R-M system from Bacillus pumilus 10, which recognizes the asymmetric 5'-CCTNAGC sequence, has been cloned, sequenced and expressed in Escherichia coli . The system comprises four adjacent, similarly oriented genes encoding two m5C MTases and two subunits of Bpu 10I ENase (34.5 and 34 kDa...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Stankevicius, K, Lubys, A, Timinskas, A, Vaitkevicius, D, Janulaitis, A
Format: Artigo
Language:Inglês
Published: Oxford University Press 1998
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147350/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9461472/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.4.1084
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