Microinjection of cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes.

We have developed a strategy for producing single copy transgenic mouse lines using Cre-loxP site specific recombination. The method is based on transient expression of the recombinase after injection of in vitro transcribed mRNA into the cytoplasm of fertilised eggs containing multiple copies of th...

Полное описание

Библиографические подробности

Опубликовано в::	Nucleic Acids Res
Главные авторы:	de Wit, T, Drabek, D, Grosveld, F
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 1998
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147291/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9421533/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.2.676
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