Random-priming in vitro recombination: an effective tool for directed evolution.

A simple and efficient method for in vitro mutagenesis and recombination of polynucleotide sequences is reported. The method involves priming template polynucleotide(s) with random-sequence primers and extending to generate a pool of short DNA fragments which contain a controllable level of point mu...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Shao, Z, Zhao, H, Giver, L, Arnold, F H
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1998
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147287/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9421535/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.2.681
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