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Chemical synthesis and characterization of branched oligodeoxyribonucleotides (bDNA) for use as signal amplifiers in nucleic acid quantification assays.

The divergent synthesis of bDNA structures is described. This new type of branched DNA contains one unique oligonucleotide, the primary sequence, covalently attached through a comb-like branching network to many identical copies of a different oligonucleotide, the secondary sequence. The bDNA comb m...

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Detalles Bibliográficos
Main Authors: Horn, T, Chang, C A, Urdea, M S
Formato: Artigo
Idioma:Inglês
Publicado: 1997
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC147109/
https://ncbi.nlm.nih.gov/pubmed/9365266
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