Insertions of mini-Tn10 transposon T-POP in Salmonella enterica sv. typhi.

We have mutagenized a clinical strain of Salmonella enterica sv. typhi with mini-transposon Tn10dTet (T-POP) to obtain conditional lethal (tetracycline-dependent) mutants with T-POP insertions upstream of essential genes. Generalized transducing phage P22 was used to introduce T-POP from a S. typhim...

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Hauptverfasser: Hidalgo, Alejandro A, Trombert, A Nicole, Castro-Alonso, J C, Santiviago, Carlos A, Tesser, Bruno R, Youderian, Philip, Mora, Guido C
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2004
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1470937/
https://ncbi.nlm.nih.gov/pubmed/15280224
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.104.026682
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