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Insertions of mini-Tn10 transposon T-POP in Salmonella enterica sv. typhi.
We have mutagenized a clinical strain of Salmonella enterica sv. typhi with mini-transposon Tn10dTet (T-POP) to obtain conditional lethal (tetracycline-dependent) mutants with T-POP insertions upstream of essential genes. Generalized transducing phage P22 was used to introduce T-POP from a S. typhim...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2004
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1470937/ https://ncbi.nlm.nih.gov/pubmed/15280224 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.104.026682 |
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