Exploiting unassigned codons in Micrococcus luteus for tRNA-based amino acid mutagenesis.
An alternative to suppression of stop codons for the biosynthetic insertion of non-natural amino acids has been developed. Micrococcus luteus , a Gram-positive bacterium, is incapable of translating at least two codons. One of these unused codons was inserted in a gene to act as a nonsense site. An...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1997
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147087/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9358183/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.22.4685 |
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