Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA.

Methylation analysis of individual cytosines in genomic DNA can be determined quantitatively by bisulphite treatment and PCR amplification of the target DNA sequence, followed by restriction enzyme digestion or sequencing. Methylated and unmethylated molecules, however, have different sequences afte...

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Bibliografiske detaljer
Main Authors: Warnecke, P M, Stirzaker, C, Melki, J R, Millar, D S, Paul, C L, Clark, S J
Format: Artigo
Sprog:Inglês
Udgivet: 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC147052/
https://ncbi.nlm.nih.gov/pubmed/9336479
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