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Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.

A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleot...

詳細記述

保存先:
書誌詳細
主要な著者: Kinoshita, Y, Nishigaki, K, Husimi, Y
フォーマット: Artigo
言語:Inglês
出版事項: 1997
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC146945/
https://ncbi.nlm.nih.gov/pubmed/9278501
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