Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.

A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.

Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Kinoshita, Y, Nishigaki, K, Husimi, Y
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146945/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9278501/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.18.3747
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