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Cleavage of collagen RNA transcripts by hammerhead ribozymes in vitro is mutation-specific and shows competitive binding effects.

We report here the in vitro use of hammerhead ribozymes as an approach to the gene therapy of osteogenesis imperfecta (OI). Our strategy for the treatment of this dominant genetic disorder is based on selective reduction of the level of the mRNA transcripts from the mutant allele. We studied the in...

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Hlavní autoři: Grassi, G, Forlino, A, Marini, J C
Médium: Artigo
Jazyk:Inglês
Vydáno: 1997
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC146924/
https://ncbi.nlm.nih.gov/pubmed/9254703
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