Re-usable DNA template for the polymerase chain reaction.

DNA covalently bound to an uncharged nylon membrane was used for consecutive amplifications of several different genes by PCR. Successful PCR amplifications were obtained for membrane-bound genomic and plasmid DNA. Membrane-bound genomic DNA templates were re-used at least 15 times for PCR with spec...

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Bibliographic Details
Main Authors: Sheikh, S N, Lazarus, P
Format: Artigo
Language:Inglês
Published: 1997
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC146921/
https://ncbi.nlm.nih.gov/pubmed/9254716
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