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Microamplification of specific chromosome sequences; an improved method for genome analysis.

An improved method was developed for microdissection and cloning of metaphase as well as pachytene chromosomes. The protocol incorporates efficient ligation of chromosomal DNA with linker adaptors, abolishment of microcloning steps and the reduction of micromanipulation. The threshold for amplifying...

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Detalhes bibliográficos
Main Authors: Ponelies, N, Stein, N, Weber, G
Formato: Artigo
Idioma:Inglês
Publicado em: 1997
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC146918/
https://ncbi.nlm.nih.gov/pubmed/9254720
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