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Microamplification of specific chromosome sequences; an improved method for genome analysis.

An improved method was developed for microdissection and cloning of metaphase as well as pachytene chromosomes. The protocol incorporates efficient ligation of chromosomal DNA with linker adaptors, abolishment of microcloning steps and the reduction of micromanipulation. The threshold for amplifying...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Ponelies, N, Stein, N, Weber, G
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1997
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC146918/
https://ncbi.nlm.nih.gov/pubmed/9254720
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