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Quantitative DNA slot blot analysis: inhibition of DNA binding to membranes by magnesium ions.

Titers of wild-type and recombinant adeno-associated viruses are routinely determined by DNA slot blot analysis. The binding of viral DNA to nylon membranes was found to be inhibited by magnesium ions, which are critical components of the DNase I digestion carried out prior to slot blot analysis. Mg...

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Bibliografski detalji
Glavni autori: Kube, D M, Srivastava, A
Format: Artigo
Jezik:Inglês
Izdano: 1997
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC146885/
https://ncbi.nlm.nih.gov/pubmed/9241256
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