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A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml.

The branched DNA hybridization assay has been improved by the inclusion of the novel nucleotides, isoC and isoG, in the amplification sequences to prevent non-specific hybridization. The novel isoC, isoG-containing amplification sequences have no detectable interaction with any natural DNA sequence....

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Dades bibliogràfiques
Publicat a:Nucleic Acids Res
Autors principals: Collins, M L, Irvine, B, Tyner, D, Fine, E, Zayati, C, Chang, C, Horn, T, Ahle, D, Detmer, J, Shen, L P, Kolberg, J, Bushnell, S, Urdea, M S, Ho, D D
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1997
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146852/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9224596/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.15.2979
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