A biologically active 53 kDa fragment of overproduced alanyl-tRNA synthetase from Thermus thermophilus HB8 specifically interacts with tRNA Ala acceptor helix.

The alaS gene encoding the alanyl-tRNA synthetase (AlaRS) from Thermus thermophilus HB8 was cloned and sequenced. The gene comprises 2646 bp, corresponding to 882 amino acids, 45% of which are identical to the enzyme from Escherichia coli . The T. thermophilus AlaRS was overproduced in E.coli , puri...

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Main Authors: Lechler, A, Martin, A, Zuleeg, T, Limmer, S, Kreutzer, R
Format: Artigo
Jezik:Inglês
Izdano: 1997
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC146809/
https://ncbi.nlm.nih.gov/pubmed/9207019
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