A new technique for the characterization of long-range tertiary contacts in large RNA molecules: insertion of a photolabel at a selected position in 16S rRNA within the Escherichia coli ribosome.

A new approach for inserting a photo-label at a selected position within the long ribosomal RNA molecules has been developed. The Escherichia coli 16S rRNA was cleaved at a single internucleotide bond, 1141-1142, with RNase H in the presence of a complementary chimeric oligonucleotide. 4-Thiouridine...

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Detalhes bibliográficos
Main Authors: Baranov, P V, Dokudovskaya, S S, Oretskaya, T S, Dontsova, O A, Bogdanov, A A, Brimacombe, R
Formato: Artigo
Idioma:Inglês
Publicado em: 1997
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC146764/
https://ncbi.nlm.nih.gov/pubmed/9171076
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