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Fractionation, phosphorylation and ligation on oligonucleotide microchips to enhance sequencing by hybridization.

Oligonucleotide microchips are manufactured by immobilizing presynthesized oligonucleotides within 0.1 x 0.1 x 0.02 mm or 1 x 1 x 0.02 mm polyacrylamide gel pads arranged on the surface of a microscope slide. The gel pads are separated from each other by hydrophobic glass spacers and serve as a kind...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Dubiley, S, Kirillov, E, Lysov, Y, Mirzabekov, A
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1997
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146758/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9171075/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.12.2259
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