Glutathione S-transferase fusion proteins as an affinity reagent for rapid isolation of specific sequence directly from genomic DNA.

We describe a DNA binding assay for isolation of specific sequence(s) recognized by protein of interest directly from genomic or cosmid DNA. In our assay, the protein is fused to the glutathione-S-transferase and bound to glutathione-Sepharose beads. Then the immobilized fusion protein can be used t...

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Bibliographic Details
Main Authors: Majka, J, Jakimowicz, D, Zarko-Postawka, M, Zakrzewska-Czerwinska, J
Format: Artigo
Language:Inglês
Published: 1997
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC146755/
https://ncbi.nlm.nih.gov/pubmed/9171112
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