Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (Ms-SNuPE).

We have developed a rapid quantitative method (Ms-SNuPE) for assessing methylation differences at specific CpG sites based on bisulfite treatment of DNA followed by single nucleotide primer extension. Genomic DNA was first reacted with sodium bisulfite to convert unmethylated cytosine to uracil whil...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Gonzalgo, M L, Jones, P A
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1997
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146734/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9171109/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.12.2529
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