A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR.

A rapid method is described to efficiently perform site-directed mutagenesis based on overlap extension polymerase chain reaction (OE-PCR). Two template DNA molecules in different orientations relative to only one universal primer were amplified in parallel. By choosing a high dilution of mutagenic...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Urban, A, Neukirchen, S, Jaeger, K E
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146699/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9153325/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.11.2227
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