Direct DNA sequence determination from total genomic DNA.
It is possible to perform a combined amplification and sequencing reaction ('DEXAS') directly from complex DNA mixtures by using two thermostable DNA polymerases, one that favours the incorporation of deoxynucleotides over dideoxynucleotides, and one which has a decreased ability to discriminate bet...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1997
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146698/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9115374/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.10.2032 |
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