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Preparation and characterization of a uniformly 2 H/ 15 N-labeled RNA oligonucleotide for NMR studies.

An RNA oligonucleotide that contains the binding site for Escherichia coli ribosomal protein S8 was prepared with uniform 15N isotopic enrichment and uniform deuterium enrichment at all non-exchangeable sites using enzymatic methods. The RNA binding site, which contains 44 nt, forms a hairpin in sol...

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Udgivet i:Nucleic Acids Res
Principais autores: Nikonowicz, E P, Michnicka, M, Kalurachchi, K, DeJong, E
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146606/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9060434/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.7.1390
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