Simple and rapid preparation of plasmid template by a filtration method using microtiter filter plates.

We developed a new simple high-throughput plasmid DNA extraction procedure, based on a modified alkaline lysis method, using only one 96-well microtiter glassfilter plate. In this method, cell harvesting, lysis by alkaline and plasmid purification are performed on only one microtiter glassfilter pla...

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Détails bibliographiques

Publié dans:	Nucleic Acids Res
Auteurs principaux:	Itoh, M, Carninci, P, Nagaoka, S, Sasaki, N, Okazaki, Y, Ohsumi, T, Muramatsu, M, Hayashizaki, Y
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 1997
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146577/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9092649/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.6.1315
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