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A reliable external control for ribonuclease protection assays.

A method is described for generating an external spiked human RNA control to enhance the reliability of assessment of gene expression in tumour extracts. Spiking with an external standard RNA controls for all subsequent steps of analysis on a lane by lane basis and allows for uniform comparison of t...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Scott, P A, Smith, K, Bicknel, R, Harris, A L
Format: Artigo
Language:Inglês
Published: Oxford University Press 1997
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146574/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9092644/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.6.1305
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