Identification of RNA-binding proteins that regulate FGFR2 splicing through the use of sensitive and specific dual color fluorescence minigene assays

We have developed a series of fluorescent splicing reporter minigenes for the establishment of cell-based screens to identify splicing regulatory proteins. A key technical advance in the application of these reporters was the use of two different fluorescent proteins: EGFP and monomeric Red Fluoresc...

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Detalhes bibliográficos
Main Authors: Newman, Emily A., Muh, Stephanie J., Hovhannisyan, Ruben H., Warzecha, Claude C., Jones, Richard B., Mckeehan, Wallace L., Carstens, Russ P.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2006
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Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1464843/
https://ncbi.nlm.nih.gov/pubmed/16603716
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.34906
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