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Template-directed dye-terminator incorporation (TDI) assay: a homogeneous DNA diagnostic method based on fluorescence resonance energy transfer.

A new method for DNA diagnostics based on template-directed primer extension and detection by fluorescence resonance energy transfer is described. In this method, amplified genomic DNA fragments containing polymorphic sites are incubated with a 5'-fluorescein-labeled primer (designed to hybridi...

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書目詳細資料
Main Authors: Chen, X, Kwok, P Y
格式: Artigo
語言:Inglês
出版: 1997
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC146426/
https://ncbi.nlm.nih.gov/pubmed/9016564
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