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Deoxy- and dideoxynucleotide discrimination and identification of critical 5' nuclease domain residues of the DNA polymerase I from Mycobacterium tuberculosis.

The DNA polymerase I (PolI) from Mycobacterium tuberculosis (Mtb) was overproduced in Escherichia coli as an enzymatically active, recombinant protein with or without an N-terminal His-tag. The proteins catalysed both the DNA polymerisation of homo- and heteropolymer template-primers and the 5'-3' e...

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Publicat a:Nucleic Acids Res
Autors principals: Mizrahi, V, Huberts, P
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1996
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146340/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016652/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.24.4845
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