T7 RNA polymerase cannot transcribe through a highly knotted DNA template.
The ability of T7 RNA polymerase to transcribe a plasmid DNA in vitro in its linear, supercoiled, relaxed and knotted forms was analysed. Similar levels of transcription were found on each template with the exception of plasmids showing varying degrees of knotting (obtained using stoichiometric amou...
Shranjeno v:
| izdano v: | Nucleic Acids Res |
|---|---|
| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1996
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146338/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016657/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.24.4890 |
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