Rapid analysis of DNA methylation using new restriction enzyme sites created by bisulfite modification.
Bisulfite converts non-methylated cytosine in DNA to uracil leaving 5-methylcytosine unaltered. Here, predicted changes in restriction enzyme sites following reaction of genomic DNA with bisulfite and amplification of the product by the polymerase chain reaction (PCR) were used to assess the methyla...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1996
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146335/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9016683/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.24.5058 |
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