Multiplex Cre/lox recombination permits selective site-specific DNA targeting to both a natural and an engineered site in the yeast genome.
Variant lox sites having an altered spacer region (heterospecific lox sites) are not proficient for Cre-mediated recombination with the canonical 34 bp loxP site, but can recombine with each other. By placing different heterospecific lox sites at different genomic locations, Cre can catalyze indepen...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autor principal: | |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1996
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146311/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8972844/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.23.4608 |
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