Improved large scale culture of Methylophilus methylotrophus for 13C/15N labeling and random fractional deuteration of ribonucleotides.

Isotopic labeling of RNA with 13C and 15N has become a routine procedure in structural studies by NMR spectroscopy. The methodology in this paper describes the random fractional deuteration of RNA using the obligate methylotropic bacterium, Methylophilus methylotrophus. This bacterium was grown usin...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Batey, R T, Cloutier, N, Mao, H, Williamson, J R
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1996
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146305/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8972874/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.23.4836
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