Directional random oligonucleotide primed (DROP) global amplification of cDNA: its application to subtractive cDNA cloning.

We describe a method of global PCR amplification of cDNA such that the strand sense is maintained. The products of this process are random primed fragments ranging in size from 100 to 500 bp which facilitates uniform PCR amplification of total cDNA. Directional incorporation of a T7 RNA polymerase i...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Hampson, I N, Hampson, L, Dexter, T M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146298/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8972873/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.23.4832
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