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Different thermostabilities of FLP and Cre recombinases: implications for applied site-specific recombination.

Genomic manipulations using site-specific recombinases rely on their applied characteristics in living systems. To understand their applied properties so that they can be optimally deployed, we compared the recombinases FLP and Cre in two assays. In both Escherichia coli and in vitro, FLP shows a di...

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Udgivet i:Nucleic Acids Res
Principais autores: Buchholz, F, Ringrose, L, Angrand, P O, Rossi, F, Stewart, A F
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146240/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8932381/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.21.4256
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