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Rapid preparation of single stranded DNA from PCR products by streptavidin induced electrophoretic mobility shift.

Streptavidin induced electrophoretic mobility shift was used to prepare single stranded (ss) DNA amplified with the polymerase chain reaction in the presence of a biotinylated and a non-biotinylated primer. A variety of denaturing conditions, including incubation at 95 degrees C in 50% formamide can...

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書目詳細資料
發表在:Nucleic Acids Res
主要作者: Pagratis, N C
格式: Artigo
語言:Inglês
出版: Oxford University Press 1996
主題:
在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146126/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8836196/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.18.3645
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