Rapid preparation of single stranded DNA from PCR products by streptavidin induced electrophoretic mobility shift.
Streptavidin induced electrophoretic mobility shift was used to prepare single stranded (ss) DNA amplified with the polymerase chain reaction in the presence of a biotinylated and a non-biotinylated primer. A variety of denaturing conditions, including incubation at 95 degrees C in 50% formamide can...
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| 發表在: | Nucleic Acids Res |
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| 主要作者: | |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
1996
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146126/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8836196/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.18.3645 |
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