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A rapid method for detecting specific amplified PCR fragments in microtiter plates.
A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are separated from unincorporated nucleotide...
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Main Authors: | , |
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格式: | Artigo |
語言: | Inglês |
出版: |
1996
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主題: | |
在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC146076/ https://ncbi.nlm.nih.gov/pubmed/8774915 |
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