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Quantitative measurement of dihydrouridine in RNA using isotope dilution liquid chromatography-mass spectrometry (LC/MS).

A method has been developed for the microscale determination of 5,6-dihydrouridine, the most common post-transcriptional modification in bacterial and eukaryotic tRNA. The method is based on stable isotope dilution liquid chromatography-mass spectrometry (LC/MS) using [1,3-15N2]dihydrouridine and [1...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Dalluge, J J, Hashizume, T, McCloskey, J A
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 1996
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146067/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8774907/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.16.3242
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