Positive selection of novel peroxisome biogenesis-defective mutants of the yeast Pichia pastoris.

We have developed two novel schemes for the direct selection of peroxisome-biogenesis-defective (pex) mutants of the methylotrophic yeast Pichia pastoris. Both schemes take advantage of our observation that methanol-induced pex mutants contain little or no alcohol oxidase (AOX) activity. AOX is a pe...

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Bibliografiska uppgifter
Huvudupphovsmän: Johnson, M A, Waterham, H R, Ksheminska, G P, Fayura, L R, Cereghino, J L, Stasyk, O V, Veenhuis, M, Kulachkovsky, A R, Sibirny, A A, Cregg, J M
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1999
Ämnen:
Research Article
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1460572/
https://ncbi.nlm.nih.gov/pubmed/10101164
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