Gene targeting in rat embryo fibroblasts promoted by the polyomavirus large T antigen.
We used the recombination-promoting activity of the polyomavirus large T antigen (T-ag) to increase the frequency of gene targeting in rat fibroblasts. We constructed a cell line carrying a functional polyomavirus replication origin and a transformation-defective middle T-ag oncogene. The structure...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1996
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145918/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8668528/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.11.1999 |
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