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Preparation of biologically active Ascaris suum mitochondrial tRNAMet with a TV-replacement loop by ligation of chemically synthesized RNA fragments.

Ascaris suum mitochondrial tRNA Met lacking the entire T stem was prepared by enzymatic ligation of two chemically synthesized RNA fragments. The synthetic tRNA could be charged with methionine by A.suum mitochondrial extract, although the charging activity was considerably low compared with that of...

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Библиографические подробности
Главные авторы: Ohtsuki, T, Kawai, G, Watanabe, Y, Kita, K, Nishikawa, K, Watanabe, K
Формат: Artigo
Язык:Inglês
Опубликовано: 1996
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC145695/
https://ncbi.nlm.nih.gov/pubmed/8604307
Метки: Добавить метку
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