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Chip PCR. II. Investigation of different PCR amplification systems in microbabricated silicon-glass chips.

We examined PCR in silicon dioxide-coated silicon-glass chips (12 microl in volume with a surface to volume ratio of approximately 17.5 mm(2)/microl) using two PCR reagent systems: (i) the conventional reagent system using Taq DNA polymerase; (ii) the hot-start reagent system based on a mixture of T...

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Udgivet i:Nucleic Acids Res
Principais autores: Cheng, J, Shoffner, M A, Hvichia, G E, Kricka, L J, Wilding, P
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1996
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC145641/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8628666/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.2.380
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