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A simple method for the preparation of pure and active gamma-globulin-ferritin conjugates using glutaraldehyde

A method is described for coupling γ-globulin to ferritin using glutaraldehyde. Isolation of the conjugate is achieved by ammonium sulphate precipitation. The conjugate is free from uncoupled ferritin or γ-globulin and the coupled antibody retains full activity. Preparation of pure active conjugate...

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Detalhes bibliográficos
Main Authors: Siess, E., Wieland, O., Miller, F.
Formato: Artigo
Idioma:Inglês
Publicado em: 1971
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1455871/
https://ncbi.nlm.nih.gov/pubmed/4137515
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