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Precipitin reactions of the C1q component of complement with aggregated γ-globulin and immune complexes ingeldiffusion

A gel diffusion method for demonstrating precipitin reactions of C1q with aggregated γ-globulin and immune complexes is described. Optimal precipitin lines were found to occur with 0.6 per cent agarose in 0.01 M EDTA at pH 7.2 and ionic strength 0.1. Reduction and alkylation of γ-globulin aggregates...

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Bibliografiske detaljer
Main Authors: Agnello, V., Winchester, R. J., Kunkel, H. G.
Format: Artigo
Sprog:Inglês
Udgivet: 1970
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1455666/
https://ncbi.nlm.nih.gov/pubmed/4098878
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